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fxii antibody cl20055  (Cedarlane)


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    Cedarlane fxii antibody cl20055
    Factor XII-W268R displays spontaneous activity during protein expression. A, Western blotting of expression medium under reducing (R) conditions. The closed arrow indicates the FXIIa light chain. The image is representative of three separate experiments. B, chromogenic substrate assay for FXIIa activity in expression medium. C, quantification of FXIIa activity in expression medium. Substrate conversion (linear) was monitored in the first 20 min of the assays and fitted to a standard curve of αFXIIa in expression medium. D, chromogenic substrate assay for plasma prekallikrein activator activity in expression medium. E, effects of PPACK, C1-INH, or antibody OT2 on FXIIa activity in expression medium. F, FXIIa–C1-INH complex formation in expression medium. After incubation with C1-INH, a sigmoidal 4PL fit model was used to interpolate concentrations from a standard curve. The figure panels represent means and S.D. of three repeated experiments.
    Fxii Antibody Cl20055, supplied by Cedarlane, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+t+cell+serum/pmc06956518-499-2-7?v=Cedarlane
    Average 91 stars, based on 13 article reviews
    fxii antibody cl20055 - by Bioz Stars, 2026-08
    91/100 stars

    Images

    1) Product Images from "A mutation in the kringle domain of human factor XII that causes autoinflammation, disturbs zymogen quiescence, and accelerates activation"

    Article Title: A mutation in the kringle domain of human factor XII that causes autoinflammation, disturbs zymogen quiescence, and accelerates activation

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA119.009788

    Factor XII-W268R displays spontaneous activity during protein expression. A, Western blotting of expression medium under reducing (R) conditions. The closed arrow indicates the FXIIa light chain. The image is representative of three separate experiments. B, chromogenic substrate assay for FXIIa activity in expression medium. C, quantification of FXIIa activity in expression medium. Substrate conversion (linear) was monitored in the first 20 min of the assays and fitted to a standard curve of αFXIIa in expression medium. D, chromogenic substrate assay for plasma prekallikrein activator activity in expression medium. E, effects of PPACK, C1-INH, or antibody OT2 on FXIIa activity in expression medium. F, FXIIa–C1-INH complex formation in expression medium. After incubation with C1-INH, a sigmoidal 4PL fit model was used to interpolate concentrations from a standard curve. The figure panels represent means and S.D. of three repeated experiments.
    Figure Legend Snippet: Factor XII-W268R displays spontaneous activity during protein expression. A, Western blotting of expression medium under reducing (R) conditions. The closed arrow indicates the FXIIa light chain. The image is representative of three separate experiments. B, chromogenic substrate assay for FXIIa activity in expression medium. C, quantification of FXIIa activity in expression medium. Substrate conversion (linear) was monitored in the first 20 min of the assays and fitted to a standard curve of αFXIIa in expression medium. D, chromogenic substrate assay for plasma prekallikrein activator activity in expression medium. E, effects of PPACK, C1-INH, or antibody OT2 on FXIIa activity in expression medium. F, FXIIa–C1-INH complex formation in expression medium. After incubation with C1-INH, a sigmoidal 4PL fit model was used to interpolate concentrations from a standard curve. The figure panels represent means and S.D. of three repeated experiments.

    Techniques Used: Activity Assay, Expressing, Western Blot, Incubation



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    Factor XII-W268R displays spontaneous activity during protein expression. A, Western blotting of expression medium under reducing (R) conditions. The closed arrow indicates the FXIIa light chain. The image is representative of three separate experiments. B, chromogenic substrate assay for FXIIa activity in expression medium. C, quantification of FXIIa activity in expression medium. Substrate conversion (linear) was monitored in the first 20 min of the assays and fitted to a standard curve of αFXIIa in expression medium. D, chromogenic substrate assay for plasma prekallikrein activator activity in expression medium. E, effects of PPACK, C1-INH, or antibody OT2 on FXIIa activity in expression medium. F, FXIIa–C1-INH complex formation in expression medium. After incubation with C1-INH, a sigmoidal 4PL fit model was used to interpolate concentrations from a standard curve. The figure panels represent means and S.D. of three repeated experiments.
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    Factor XII-W268R displays spontaneous activity during protein expression. A, Western blotting of expression medium under reducing (R) conditions. The closed arrow indicates the FXIIa light chain. The image is representative of three separate experiments. B, chromogenic substrate assay for FXIIa activity in expression medium. C, quantification of FXIIa activity in expression medium. Substrate conversion (linear) was monitored in the first 20 min of the assays and fitted to a standard curve of αFXIIa in expression medium. D, chromogenic substrate assay for plasma prekallikrein activator activity in expression medium. E, effects of PPACK, C1-INH, or antibody OT2 on FXIIa activity in expression medium. F, FXIIa–C1-INH complex formation in expression medium. After incubation with C1-INH, a sigmoidal 4PL fit model was used to interpolate concentrations from a standard curve. The figure panels represent means and S.D. of three repeated experiments.
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    Factor XII-W268R displays spontaneous activity during protein expression. A, Western blotting of expression medium under reducing (R) conditions. The closed arrow indicates the FXIIa light chain. The image is representative of three separate experiments. B, chromogenic substrate assay for FXIIa activity in expression medium. C, quantification of FXIIa activity in expression medium. Substrate conversion (linear) was monitored in the first 20 min of the assays and fitted to a standard curve of αFXIIa in expression medium. D, chromogenic substrate assay for plasma prekallikrein activator activity in expression medium. E, effects of PPACK, C1-INH, or antibody OT2 on FXIIa activity in expression medium. F, FXIIa–C1-INH complex formation in expression medium. After incubation with C1-INH, a sigmoidal 4PL fit model was used to interpolate concentrations from a standard curve. The figure panels represent means and S.D. of three repeated experiments.
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    Image Search Results


    Factor XII-W268R displays spontaneous activity during protein expression. A, Western blotting of expression medium under reducing (R) conditions. The closed arrow indicates the FXIIa light chain. The image is representative of three separate experiments. B, chromogenic substrate assay for FXIIa activity in expression medium. C, quantification of FXIIa activity in expression medium. Substrate conversion (linear) was monitored in the first 20 min of the assays and fitted to a standard curve of αFXIIa in expression medium. D, chromogenic substrate assay for plasma prekallikrein activator activity in expression medium. E, effects of PPACK, C1-INH, or antibody OT2 on FXIIa activity in expression medium. F, FXIIa–C1-INH complex formation in expression medium. After incubation with C1-INH, a sigmoidal 4PL fit model was used to interpolate concentrations from a standard curve. The figure panels represent means and S.D. of three repeated experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: A mutation in the kringle domain of human factor XII that causes autoinflammation, disturbs zymogen quiescence, and accelerates activation

    doi: 10.1074/jbc.RA119.009788

    Figure Lengend Snippet: Factor XII-W268R displays spontaneous activity during protein expression. A, Western blotting of expression medium under reducing (R) conditions. The closed arrow indicates the FXIIa light chain. The image is representative of three separate experiments. B, chromogenic substrate assay for FXIIa activity in expression medium. C, quantification of FXIIa activity in expression medium. Substrate conversion (linear) was monitored in the first 20 min of the assays and fitted to a standard curve of αFXIIa in expression medium. D, chromogenic substrate assay for plasma prekallikrein activator activity in expression medium. E, effects of PPACK, C1-INH, or antibody OT2 on FXIIa activity in expression medium. F, FXIIa–C1-INH complex formation in expression medium. After incubation with C1-INH, a sigmoidal 4PL fit model was used to interpolate concentrations from a standard curve. The figure panels represent means and S.D. of three repeated experiments.

    Article Snippet: Polyclonal affinity-purified FXII antibody CL20055 was from Cederlane (Burlington, Canada).

    Techniques: Activity Assay, Expressing, Western Blot, Incubation